Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer.
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چکیده
منابع مشابه
Serum alkaline phosphatase measured by using thymol blue monophosphate and a bichromatic analyzer.
Thymol blue monophosphate is evaluated as a substrate for use in kinetic bichromatic analysis of serum alkaline phosphatase activity. The effect of abnormally high concentrations of hemoglobin, bilirubin, or chylomicrons is negligible. The expected range for ambulatory adults established by assaying sera from 206 blood donors, was 12-44 U/lliter at 37 degrees C. Comparison of the method with a ...
متن کاملSerum alkaline phosphatase: total activity and isoenzyme determinations made by use of the centrifugal fast analyzer.
We present a kinetic method in which the centrifugal analyzer is used to determine total alkaline phosphatase activity and to measure the isoenzymes in bone, liver, and the L-phenylalanine-sensitive fraction (intestine, tumor, and placenta). Measurements were made at 30#{176}C in diethanolamine buffer, with p-nitrophenylphosphate as substrate. The a!kaline phosphatase isoenzymes were separated ...
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Background: The present study was carried out on the human population of Kashmir valley to evaluate the status of the biochemical parameters of infected population. Materials and Methods: Blood samples were collected from 514 individuals, 298 (57.97%) males and 216 (42.02%) females, 187 (36.38%) were found sero-positive for human toxocariasis. Results: Alkaline phosphatase level was found highe...
متن کاملSerum Alkaline Phosphatase
phatase may reflect enzyme contributions from bone, liver, and intestine. We have investigated serum alkaline phosphatases in two siblings with hypophosphatasia. After administration of long-chain triglycerides, the major alkaline phosphatase component of their sera was shown to be of intestinal origin on the basis of inhibition by l-phenylalanine. Starch block electrophoresis suggested that th...
متن کاملAmmonium thymolphthalein monophosphate as a new substrate for alkaline and acid phosphatase determinations in serum.
A new substrate, ammonium thymolphthalein monophosphate, is proposed for use in alkaline and acid phosphatase determinations. The ammonium salt shares the advantages of the sodium salt, but is a more stable substrate and results in more stable working reagents. The substrate is buffered at ph 10.1 in diethanolamine for determining alkaline phosphatase activity in 5 mm, and at pH 5.9 in citrate ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1976
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/22.10.1737